Visual gene developer: a fully programmable bioinformatics software for synthetic gene optimization

Applied Bioinformatics for Gene Characterization

Optimization of conditions for gene delivery system based on PEI

Objective(s): PEI based nanoparticle (NP) due to dual capabilities of proton sponge and DNA binding is known as powerful tool for nucleic acid delivery to cells. However, serious cytotoxicity and complicated conditions, which govern NPs properties and its interactions with cells practically, hindered achievement to high transfection efficiency. Here, we have tried to optimize the properties of ...

Codon Optimization OnLine (COOL): a web-based multi-objective optimization platform for synthetic gene design

SUMMARY Codon optimization has been widely used for designing synthetic genes to improve their expression in heterologous host organisms. However, most of the existing codon optimization tools consider a single design criterion and/or implement a rather rigid user interface to yield only one optimal sequence, which may not be the best solution. Hence, we have developed Codon Optimization OnLine...

Design and bioinformatics analysis of novel biomimetic peptides as nanocarriers for gene transfer

Objective(s): The introduction of nucleic acids into cells for therapeutic objectives is significantly hindered by the size and charge of these molecules and therefore requires efficient vectors that assist cellular uptake. For several years great efforts have been devoted to the study of development of recombinant vectors based on biological domains with potential applications in gene therapy....

Optimization of a PCR Method for Detection of Lipase Gene in Bacillus subtilis

     Lipases catalyze hydrolysis and synthesis of triacylglycerols. These enzymes,especially the microbial ones, have wide commercial and industrial usage. Bacillus subtilis <span style="color: ...